Fil's tree is the first Ori comes across in the Sunken Glades. When Ori first discovers it, the Spirit Tree comments on how Fil and others like him perished in the wake of Kuro's attack. Fil's tree passes on the Wall Jump ability.
Ano's tree can be found in the depths of Hollow Grove. Strong and courageous, Ano gives Ori the ability to Charge Flame.
Leru's tree is found in Gumo's Hideout. With the ability to cut through the air, her light allows Ori to learn Double Jump. Through further upgrades on the Ability Tree, Ori can turn this into a Triple Jump.
Tatsu's ability used to Climb is a trait she used to explore the mountains high atop Sorrow Pass. She later lost hope when her brother's torch faded and she fell to the mists of Misty Woods where her tree rests today.
A spirit who was once friends with Naru in her youth, Eki will grant the power to Dash along the ground. After the death of Naru's father forced her to leave, Eki attempted to find her but could not, eventually succumbing and turning into a Tree in the Black Root Burrows.
- Eki and Sol's trees are only found in the Definitive Edition of the game. The two Spirit Guardians that Naru plays with in her youth both use the abilities that they later give Ori, implying that they are Eki and Sol. With Ori and Fil, both of them are the only named Spirit Guardians that can be seen in game.